RED BLOOD CELL SENESCENCE IN INFLAMMATORY CHRONIC DISEASES

STAMPONE ROCIO; PACINI ANTONELLA; ENSINCK ALEJANDRA; TANNO FEDERICO ; DINATALE BRENDA ; LEIVA RODOLFO; RAMOS KARINA ; BESSONE FERNANDO; REGGIARDO M. VIRGINIA; PÉREZ ANA ROSA; COTORRUELO CARLOS; VILLAR SILVINA

Mar del plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Chronic inflammatory diseases, regardless of their nature, could impactvarious physiological processes, including the senescence ofblood cells. While it is known that the average lifespan of red bloodcells (RBCs) is 120 days, there is evidence to support that chronicinflammatory processes could accelerate the erythrocyte aging.Thus, it is possible that RBCs may have a shorter half-life in pathologieslike Chagas disease (CD) and autoimmune hepatitis (AIH). Thisstudy aims to identify changes in aging markers of RBCs, such as anincrease in autologous IgG antibodies on the RBCs membrane thatbind to band 3, stimulating C3b deposition and erythrophagocytosis,and a decrease in the expression of CD47, a transmembrane proteinwhich acts as a “don’t eat me” signal. In both cases, the removalof RBCs by macrophages is promoted. For this, we recruited individualsinfected with Trypanosoma Cruzi who developed Chronic ChagasCardiomyopathy (CCC, n=7) and those without cardiac pathology(Indeterminate form (IND), n=18), as well as patients with AIH(n=10). Healthy volunteers were matched according to sex and age(Co, n=10). Flow cytometry has been applied for detection of CD47and RBCs-bound IgG. CD47 expression was lower in both AIH, INDvs. Co, as indicated by the Median Fluorescence Intensity values(*p