INVESTIGADORES
MASSA Gabriela Alejandra
congresos y reuniones científicas
Título:
Evidence of recombinant strains of Potato virus Y (PVY) in Argentina
Autor/es:
COLAVITA MONICA LILIANA; MARCOS LANCIA; FEINGOLD SERGIO ENRIQUE; MASSA GABRIELA ALEJANDRA
Lugar:
Arrail D´Ajuda
Reunión:
Conferencia; 11th Solanacea Conference (SOL2014).; 2014
Resumen:
Potato Virus Y (PVY) is one of the
most common disease agents of potato production worldwide. Severe infections
can cause losses from 40 to 70 %. Historically PVY isolates have
been divided into three groups of strains PVYO, PVYN and PVYC,
based on their pathology in Nicotiana tabacum cv. Samsun, Solanum tuberosum Group
Tuberosum and Physalis floridana. Two main recombinant types were reported
within the necrotic PVYN group: PVYN with necrotic
symptoms in tubers (named PVYNTN) and PVYN Wilga (named
PVYNW). PVYNTN, is capable of inducing tuber necrosis, and this
symptom is often referred to as potato tuber necrotic ringspot disease (PTNRD).
In
this work, forty-six isolates of PVY selected from tubers
with PNTRD symptoms and from plants infected only with PVY from fields of
different regions of Argentina,
were biologically and serologically characterized in order to identify the
occurrence of recombinant PVY strains. Out of the 46 PVY isolates studied, 41 (89.6%) showed necrosis in tobacco
as PVYN, 5 induced mosaic symptomstypical to PVYC
or PVYO strains and 33
isolates produced PTNRD symptoms in cv. Calén INTA. Analyseswith monoclonal antibodies identified three strains PVYN,
PVYO and PVYC, 32 isolates with concordance in symptoms and
serology data and 14 isolates with some inconsistence between both
determinations. Sequences of previously
recombined hot spot regions of the genome were further investigated in five
selected isolates (ST11,
RCA5a, RCA6, RCA14b and SSF6) with conflicting behavior
and compared to PVYN
(N605, X97895) and PVYO (O- 139, U09509) reference genomes.
Isolates with one, two and three recombination points were identified
among these and also in typical PVYN and PVYO strains (SSF18 and
RCA5b) included in the study. This result confirms
the occurrence of
recombined PVY in Argentina
for the first time, and extends the discussion on the appropriate
classification of PVY viruses and the role of recombination as a mechanism of
viral evolution.