“Potato rough dwarf virus (PRDV) and Potato virus P (PVP) are strains of the same Carlavirus”

MASSA G, SEGRETIN M.E, RIERO F, COLAVITA M, BRAVO-ALMONACID F Y FEINGOLD S.

Pinamar, Buenos Aires

Congreso; SAIB, XLI Annual Meeting; 2005

Immunological evidences indicate a high degree of similarity between PRDV (Carlavirus, Argentina) and PVP (Carlavirus, Brazil). The objective of this work is to determine the relationship between PRDV and PVP by means of biological and molecular comparison. A greenhouse experiment was carried out involving the infection on 21 differential host plants along with 7 commercial potato varieties. Symptoms we recorded visually and virus presence was assessed by  DAS-ELISA. Biological results show  small differences in symptoms on host plants. N.  occidentalis, presented slightly different symptoms between both viruses. Calén INTA was not infected with PVP and Sierra Volcán INTA and Primicia INTA had differences in secondary symptoms. We have cloned and sequenced genetic regions of the replicase (RG), coat protein (CP) and 11kDa protein genes of PRDV and PVP The CP showed 100% identity, 97% identity in 11 kDa protein and 93% identity in  RG. These results along with  PRDV and PVP coupled in the same clade of a Carlaviridae phylogenetic tree, suggest that PRDV and PVP are strains of the same virus.