Novel supramolecular delivery system for Amphotericin B

PINEDA JUAN CRUZ; ARRIAGA MARIA EMILIA; FERNÁNDEZ MARIANA A.; RAQUEL V. VICO

Córdoba

Congreso; LI Reunión Anual SAB; 2023

Sociedad Argentina de Biofisica

Systemic infections caused by various types of fungi are becoming a current issue in the modern world, to the extent that in 2022, the WHO (World Health Organization) published its first list of fungi that represent a risk to human health. The rational design of drug delivery approaches leveraging supramolecular chemistry (i.e.,‘‘chemistry beyond the molecule’’) hasgarnered significant interest in recent years toward improving therapeutics. [1]In this work, we obtain and characterize a supramolecular drug delivery system (SDDS) for Amphotericin B (AmB) based on niosomes, vesicles composed of non-ionic surfactants.[2] Niosomes are a novel vesicular system that still requires research but hold great promise for the future as they are cost-effective, versatile, and biocompatible. AmB is a broad-spectrum polyene antibiotic widely used to treat candidiasis and leishmaniasis.[3,4] AmB has low water solubility, which imposes significant challenges for its administration. The niosomes we prepared comprise Tween 80 and Span 80 [5], both widely used in the pharmaceutical and cosmetic industries as excipients, with established safety. Our investigation let us propose these SDDS as an alternative for the transport and administration of AmB. The SDDS was characterized using UV-Vis spectroscopy, circular dichroism, dynamic light scattering, differential scanning calorimetry, and transmission electron microscopy. Studies over time demonstrated high stability. Also, the noisome-AmB formulation can be lyophilized and redispersed, conserving the properties of the system. Finally, the hemolysis test showed a low hemolytic capacity compared to the most used formulation of AmB.