COMPARATIVE ASSESSMENT OF AZITHROMYCIN INDUCED DYSBIOSIS IN MALE AND FEMALE C57BL7 AND FOXP3-GFP MICE.

VIRGINIA ALEJANDRA PIQUERAS; JUAN IGNACIO GIRARDI ; AGUSTINA JULIANA ERREA; MARTIN RUMBO ; SILVIA GRACIELA CORREA

San Luis

Congreso; LXXI Reunión Anual de la Sociedad Argentina de Inmunología; 2023

SAI

The intestinal microbiota is a complex community of microorganisms that plays afundamental role in the immune competence of the host. Exposure to antibioticsis one of the most common causes of disruption to this community, known asdysbiosis, and is associated with both short-term and long-term diseases. We arestudying the effects of the acute administration of Azithromycin (AZM), a widelyprescribed antibiotic in our environment, on the regulatory circuits of intestinalimmunity. Our objective is to characterize the dysbiosis in male and femaleC57BL6 and Foxp3-GFP mice after 5 days of AZM administration (50 mg/kg/day)in the drinking water, by measuring bacterial density and the activation of theTLR4 and TLR5 NF-kB pathway. Fecal samples were collected daily or on daysD0 (before AZM), D3, and D7 (2 days after AZM administration ended). We usedthe DNA intercalator SyBR Green, count beads, and flow cytometry to determinebacterial density, and the HEK BLUE TLR4 and TLR5 reporters cells lines and acolorimetric assay to measure TLR4 and TLR5 activity. We observed a decreasein bacterial density as early as 24 h after AZM intake in both strains and sexes,which was significant on D3 in female Foxp3-GFP mice (p