Capillary electrophoresis and multivariate techniques for the analysis of silver/carbon hybrid nanoparticles

SPRINGER, VALERIA; ARROYAVE, J.M.; OLIVERA, LEVI; FRAGOSO, WALLACE; CENTURIÓN, M.E.

Mendoza

Congreso; 24th Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2018

LACE

In order to improve the properties of different nanomaterials or provide a synergistic effect, hybrid nanoparticles consisting of two or more types of individual nanocomponents are emerging. Currently, this type of synthesis and its applications are being investigated [1,2]. In this work an environmentally friendly method of sequential synthesis of silver/carbon hybrid nanoparticles (AgCNPs) was developed by using glucose as reducing and capping agent. For this purpose, silver nanoparticles (AgNPs) were initially synthesized according to González Fá et al. [3]. In a second step, the colloidal suspension was subjected to a microwave treatment and carbon dots (CDs) were generated leading to the AgCNPs. Additionally, physical mixtures of the individual nanoparticles (AgNPs and CDs) were made in order to compare with the synthesis of the hybrid nanoparticles. Both individual and hybrid nanoparticles were characterized by TEM, IR, XRD, DSC, dynamic light scattering (DLS) and capillary electrophoresis (CE). Special attention was given to CE due to this technique is suitable for nanoparticles characterization based on the relationship between the electrophoretic mobility and nanoparticle size and/or surface charge density. Here, the obtained nanoparticles were analyzed by CE and multivariate analysis. Under the optimal conditions (20 mM sodium tetraborate - 20 mM sodium dodecyl sulfate at pH=8.5; applied voltage= 20 kV; hydrodynamic injection= 0.8 psi, 20 s) the nanoparticles were separated within a total time less than 25 min. Then, PARAFAC algorithm was applied to analyze the obtained data over the wavelength range from 200 to 500 nm. As preliminary conclusions, we demonstrated the combined use of CE and multivariate methodology for the analysis of AgNPs and CDs mixtures, being an important tool for further prediction of Ag/C hybrid nanoparticles composition.References1. Yang Lu Y, Lu Li N, Ping Jia L, Na Ma R, Li Jia W, Qian Tao X, Cui H, Sheng Wang H. Journal of Electroanalytical Chemistry, 2016, 781, 114?119.2. Beiraghi A, Ali Najibi-Gehraz S. Sensors and Actuators B, 2017, 253, 342?351.3. González Fá AJ, Juan A, Di Nezio MS. Analytical Letters, 2016, 50, 877?888