Chemical characterization and biological activities of the lyophilized decoction from Baccharis grisebachii

GÓMEZ J; SIMIRGIOTIS MJ; LIMA B; VILLEGAS GABUTTI CM; PAREDES JD; GAMARRA-LUQUES C; BÓRQUEZ J; LUNA L; WENDEL GH; MARIA AO; TAPIA A

MENDOZA

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2018

Sociedad de Biología de Cuyo

The decoction extract from Baccharis grisebachii is used as a digestive, to relieve gastric ulcers, cicatrizing and antiseptic in Argentine. A lyophilized decoction (LD) from the aerial parts was evaluated regarding their anti-ulcer, antioxidant and cytotoxic activities, and UHPLC-MS metabolome fingerprinting. The antioxidant properties were assayed by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The LD showed a moderated free radical scavenging activity in the DPPH assay (IC50 = 106 µg/mL) and inhibited the lipid peroxidation in erythrocytes in a 67%, at 250 µg/mL. At a dose of 750 mg/kg, the LD had the highest gastroprotective effect with a 93% inhibition of damage through a mechanism that involves NO and prostaglandins using the rat model of ethanol-induced gastric damage. On the other hand, LD does not induce cytotoxic changes on human tumor and no-tumor cell lines at concentrations assayed. Thirty-one compounds were detected and identified based on the UHPLC OT-MS including, nine correspond to flavonoids, thirteen to caffeoylquinic acid derivatives, three to coumarins and three to other different phenolic compounds. The results support that Baccharis grisebachii decoction is a valuable natural product with the potential to improve human health.Authors are grateful to CICITCA-UNSJ, UNSL, UNCuyo and U Austral de Chile.