Lactobacillus plantarum CIDCA 83114 REDUCES THE PATHOGENIC POTENTIAL OF SHIGA TOXIN (Stx)-PRODUCING Escherichia coli (STEC) O157:H7

ROMINA J FERNÁNDEZ-BRANDO; DANIELA CARBALLO; ALAN M BERNAL; FERNANDO N SOSA; MARÍA VICTORIA RAMOS; ANALÍA G ABRAHAM; GRACIELA GARROTE; MARINA S PALERMO

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias (SAIC-SAI-FAIC-SAFIS) 2022; 2022

Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 is a foodborne pathogen,which can lead to the life-threatening Hemolytic Uremic Syndrome (HUS). There is notreatment available in order to reduce HUS outcome up to date. Probiotics are livemicroorganisms that promote beneficial health effects on the host. Lactobacillus plantarumCIDCA 83114 (CIDCA 83114) was previously isolated from kefir and reduced the cytotoxicactivity of Stx on Vero cells. The aim of this work was to evaluate the effect of CIDCA 83114on the pathogenic potential of a clinically isolated STEC strain. We evaluated the inhibition ofSTEC growth by CIDCA 83114 by co-culturing both bacterial strains in ratios 1:1, 1:10 and1:100 (STEC: CIDCA 83114) and counting colony forming units by plating in specific media.Besides, we also tested the competition and exclusion exerted by CIDCA 83114 on STECadhesion to Caco-2 cells. Results were statistically analyzed by Kruskal-Wallis test.We observed that CIDCA 83114 completely inhibited STEC growth by 24 h co-culture in allthe ratios tested (p