EVALUATION OF THE ROLE OF INTERLEUKIN-10 IN THE PATHOGENESIS TRIGGERED BY STX2

FERNANDO NICOLÁS SOSA; ALAN MAURO BERNAL; YINA CARPINTERO; CAMILA CANCINO; ROMINA JIMENA FERNÁNDEZ-BRANDO; CATALINA DIRNEY ALBA-SOTO; MARINA SANDRA PALERMO; MARÍA VICTORIA RAMOS

Congreso; LXXI Reunión Anual de la Sociedad Argentina de Inmunología; 2023

Hemolytic Uremic Syndrome (HUS), characterized by hemolytic anemia, thrombocytopenia, andrenal failure, is caused by Shiga toxin (Stx)-producing E. coli (STEC). Previously we demonstratedthat IL-10 deficient (IL-10 -/- KO) Balb/c mice are protected against a Stx2 dose which is lethal forwild-type (WT) mice, in a model of intravenous (I.V) inoculation. The aim of this study was tofurther analyze the role of IL-10 in HUS, using a different Stx2 inoculation model, referred to as thesplit-dose model. WT and IL-10 -/- Balb/c mice (6-8 weeks) were injected with a lethal dose of Stx2divided into four doses, over four days. Mice were bled at days 3, 4, and 5. Percentages of PMNwere higher in IL-10 -/- mice compared to WT at days 3 (WT: 13 (9-17); KO: 47 (43-54)*; n=8;p