Transcriptome analysis of Peach-T. deformans interaction in susceptible and resistant genotypes

MARÍA VALERIA LARA; MARÍA ANGELINA NOVELLO; GABRIEL VALENTINI; MARÍA FABIANA DRINCOVICH

San José

Congreso; Reunión de la American Society of Plant Biologists (ASPB); 2019

Peach (Prunus persica) is an appreciated summer fruit. The "peach leaf disease",caused by Taphrina deformans, affects its production. Although it is controlled withfungicides, to avoid the potential negative impact on health and environment, newfarming strategies based on genetically resistant materials are needed. Here, weworked with a resistant genotype (DR) and compared it with a susceptible one(DS). Leaves inoculated with the fungus were collected at 0, 12 and 96 hpi. RNA-Seq analysis was used to identify candidate resistance genes and to dissect theearly molecular processes during the interaction in resistant and susceptiblegenotypes.T. deformans-DR interaction analysis identified 190 and 1080differentially expressed genes (DEGs) at 12 and 96 hpi with respect to 0 hpi,respectively. Using DS, 357 and 210 DEGs were identified at 12 and 96 hpi,respectively. The analysis over time indicated the most represented functionalcategories were RNA, protein, hormone, cell wall and secondary metabolisms, bioticand abiotic stress, signalling and transport.Differences regarding phytohormoneswere found between genotypes; while in DR transcripts related to jasmonatesynthesis and responses were induced; in DS those involved in its synthesis wererepressed. In DS transcripts encoding ACC synthase and oxidase were repressed;suggesting a decrease in ethylene synthesis. Conversely, in DR a transcriptencoding ACC synthase was induced at 96 hpi. With respect to auxins, therepression of transcripts involved in auxin synthesis and in auxin responsiveproteins was observed in DR. In contrast, in DS PIN5 was greatly increased.In DRat 96 hpi, it is notable the induction of plant-defense genes such as chitinases,major latex proteins-related and disease resistance proteins (NBS-, TIR-NBS- andCC-NBS-LRR). These findings provide an important basis for understanding themolecular mechanism that leads to the resistance in P. persica