Hormonomics of Prunus persica (peach) leaves inoculated with Taphrina deformans

MARIA ANGELINA NOVELLO; PETřÍK, I; KARADY, M; OKLE?ťKOVÁ, J; NOVÁK, O; VALENTINI, G.H; STRAND, M; MARIA VALERIA LARA

Rosario

Congreso; RAFV; 2023

RAFV

Peach leaf curl (PLC) is a widespread disease, caused by the fungus Taphrina deformans,characterized by leaf hyperplasia and hypertrophy. PLC leads to significant decreases inpeach yield, resulting in substantial economic losses. The objective of this study was toinvestigate the hormonal response of two peach genotypes, one susceptible (DOFI-71.043.018, DS) and another resistant (DOFI-84.364.060, DR) to the pathogen, after 12and 96 hours post inoculation (hpi) with T. deformans. Hormone profiling in leaves wasconducted by targeted metabolomics using non-selective extraction and extremelysensitive ultra-high-performance liquid chromatography-tandem mass spectrometry. Theprocedure allowed the identification of 15 species of cytokinins, 7 differentbrassinosteroids, 7 types of auxins and derivatives, 5 different jasmonates (JA) and 5species of abscisates. The precursor of ethylene, 1-aminocyclopropane-1-carboxylic acid(ACC), and salicylic acid (SA) were also quantified.Active BRs exhibited a similar response of increase in DS and DR genotypes afterinoculation while active JAs decreased in both genotypes. Additionally, in DS genotype,there was a significant increase in ACC, abscisates and auxins levels at 96 hpi comparedto 0 hpi. Therefore, this increase in auxins could be responsible for the later cellelongation and proliferation observed in curled and thickened diseased leaves. On theother hand, SA only increased in the DR at 96 hpi; thus, SA may play a crucial role in thedefense mechanism against the fungus, which conducts to the resistance to the pathogen.Moreover, SA response pattern parallels that of isochorismate synthase previouslyreported by our group.To our knowledge, this is the first hormone profiling conducted in leaves of Prunuspersica. Together, data acquired provides a significant contribution to the understandingof the diverse mechanisms operating in P. persica genotypes with differentialsusceptibility to T. deformans.