THIOREDOXIN-1 IS INVOLVED IN THE CARDIOPROTECTION MECHANISM OF VOLUNTARY EXERCISE IN MICE

GODOY OLAZAR E; PEREZ V; FRANCO RIVEROS V; GODOY OLAZAR J; LEE A; BUCHHOLZ B; CASANOVA V; CICALE E; ZAOBORNYJ T; D'ANNUNZIO V

Mar del Plata

Congreso; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2023

Voluntary exercise reduces myocardial injury caused by acute ischemia/reperfusion (I/R). However, whether thioredoxin1 overexpression(Trx1) is involved in the cardioprotection mechanism of voluntaryexercise has not been studied. The aim was to study if Trx1 andexercise share cardioprotection mechanisms. Wild type mice hearts(Wt), transgenic mice hearts overexpressing Trx1, and a dominantnegative mutant (DN) of Trx1 were used and divided in exercise (E)and sedentary group (S). Mice hearts were subjected to 30 min ofI and 120 min of R (Langendorff technique). We measured infarctsize, and transverse sections of quadriceps, gastrocnemius, soleusand myocardium muscles (H&E) and the cross-sectional area(CSA) of myofibers were measured. Also, western blotting was performedto study GSK3β phosphorylation. Data were expressed asmean±SEM and p<0.05 was considered statistically significant. n=4 each group. As we previously showed, training was confirmed byheart rate variation and exercise reduced infarct size in Wt but not inDN mice. The changes in body weight and running distance at thefourth week of training in transgenic mice were comparable with Wtmice. Nevertheless, caloric intake was higher in E groups comparedto S groups. Heart weight increased significantly with exercise andsoleus weight was greater in Wt-E and DN-E groups but were similarin S groups. There were no differences in quadriceps and gastrocnemiusweight between E and S groups. CSA results showed asimilar behavior as muscles weight (soleus: Wt-S: 1164±89, Trx1-S:1274±166, DN-S: 1102±144, Wt-E: 1665±174, Trx1-E: 1169±170,DN-E: 1326±86).Finally, GSK3β phosphorylation was increased in Egroups (Wt-I/R: 1.28±0.14, Trx1-I/R: 1.28±0.08, DN-I/R: 1.08±0.14)compared with S groups (Wt-I/R: 0.87±0.12, Trx1-I/R: 1.02±0.06,DN-I/R: 1.04±0.17). In conclusion, we found that Trx1 and exercisecould share cardioprotection mechanisms.