Diferential effect of chronic stress exposure on diabetes incidence on male and female NOD/ShiLtJ mice. Participation of gut microbiota

WYDRA, L; GONZALEZ MURANO, MR; BIANCHI, MS; WALD, MR; GENARO, AM; RUBINSTEIN MR

CABA

Congreso; REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FISIOLOGÍA. 2023; 2023

SOCIEDAD ARGENTINA DE FISIOLOGÍA

Introduction: Type 1 diabetes (T1D) is an autoimmune disease characterized by impaired insulin secretion. It has been recognized the contribution of psychosocial factors (including chronic stress exposure) in T1D in its development. Gut microbiota is the group of microorganisms (commensal, symbiotic and pathogenic) that we find in our gut. It participates in multiple functions and an association between unbalanced microbiota and several diseases, including diabetes, has been reported. NOD/ShiLtJ mice are a model for autoimmune T1D, where the animals spontaneously became diabetic. There is a marked sexual dimorphism: a higher T1D diabetes incidence in females than in males. Objectives: the aim of the present work was to study the effect of chronic stress in diabetes development and to characterize the microbiota alterations in NOD/ShiLtJ mice in females and males. Methods: Two-month old NOD/ShiLtJ mice were subject to chronic stress (CS) by the application of aleatory and unpredictable stressors. Results: females NOD/ShiLtJ mice exposed to CS showed an increase in diabetes incidence (p