TNF-INDUCED UP-REGULATION OF RAC3 EXPRESSION ELICITS A PROINFLAMMATORY PROFILE IN BREAST CANCER-ASSOCIATED ADIPOCYTES

MARÍA CECILIA LIRA; FRANCISCO DAMIÁN ROSA; IGNACIO AIELLO; MILENI SOARES MACHADO; IARA CASTELLANOS; JULIANA LOURDES BERNACCHIA; ALEJANDRA GRACIELA PALMA; MARÍA CECILIA SALAZAR GÜEMES; NATALIA PALADINO; MÓNICA ALEJANDRA COSTAS; MARÍA FERNANDA RUBIO

Congreso; SAIC (Sociedad Argentina de Investigación Clínica); 2022

The molecular mechanisms that control adipocyte be- havior in breast cancer (BrCA) context remain unclear. We previously reported that the expression of RAC3 coactivator in adipose tissue (AT) adjacent to tumors from BrCA patients, was high in 59% of the samples, showing active NF-kB in nucleus and elevated levels of pro-inflammatory cytokines (TNF and MCP1). Moreover, 3T3-L1 adipocytes (3T3-L1ad) exposed to conditioned medium (CM) from T-47D BrCA cells, exhibited higher RAC3 levels compared to CM from other tumoral and non-tumoral cells. Therefore, the aim of this work was to elucidate the role of RAC3 and the mechanism by which it is modulated in BrCA-associated adipocytes. First, we evaluated TNF and MCP1 levels by dot plot and qPCR, respectively, in 3T3-L1ad stimulated with T-47D CM or serum-free medium. Both molecules showed higher lev- els with T-47D CM (p