Pyrolirion albicans: alkaloid GC-MS profile analysis in different phenological moments

OLIMPIA LLALLA-CORDOVA; LUCIANA TALLINI; LUNA, LORENA C.; JAUME BASTIDA; GABRIELA E. FERESIN; JAVIER E. ORTIZ

San Juan

Congreso; XLI CONGRESO DE LA SOCIEDAD DE BIOLOGIA DE CUYO; 2023

SOCIEDAD DE BIOLOGIA DE CUYO

Bulbous species are perennial species that exhibit active growth and flowering in spring. They survive thanks to the underground bulb, where the reserves are accumulated during senescence. The Amaryllidaceae family is widely distributed in different regions of the neotropics and temperate zones of the world. In Peru, the genus Pyrolirion Herb has six endemic species. The chemical composition of the alkaloid extract from P. albicans bulbs collected in spring, has been recently reported. The results indicated high levels of montanine and the presence of the alkaloids Galantamine (Gal), N-Demethylgalantamine, Vittatin/Crinine, Pancracine, Sternbergine, Lycorine, Hippeastrine, 2a-Hydroxyhomolycorine in similar relative proportions, while in the leaves Gal, Clidantine, Tazettine, and Lycorine, were the most abundant. The objective was to establish if the alkaloid profile (quali- and semi-quantitatively) is affected by the phenology of the species. For this comparative analysis, P. albicans bulbs were collected in autumn (senescence period), chopped, dried, and subsequently subjected to the alkaloids extraction. Alkaloid identification and semi-quantification by GC-MS was performed using a DB5-MS column, AMDIS 2.65 program, and private alkaloid library. A calibration curve of galantamine using codeine as the internal standard (y = 38.636 x + 9.8269; y = mg Gal; x = Gal area /codeine area) was applied for the quantification, with Gal ranges of 5-900 mg/mL, and 3 injections for each Gal level. The quantity expressed as (µg Gal /100 mg dry weight). The autumn collection showed a high content of Tazettine followed by Lycorine and Sanguinine, as well as the absence of montanine. It was confirmed that, depending on the time of collection, the alkaloid profile is different. Regarding the high content of tazzetine (32.3μg/100mg) in the bulbs from the autumn collection, it could be explained considering that, at the time of senescence, the alkaloids from the aerial part (leaves) are stored in the bulbs. It is evident that phenology would greatly affect the composition and relative proportion of alkaloids in this species, indicating that the time of collection is decisive for the alkaloid of interest, whether it is montanine to collect in spring and whether it is tazzetine to collect in autumn.