INHIBITORY EFFECTS OF 6-O-PALMITOYL-L-ASCORBIC ACID (ASC16) ON Bothrops alternatus VENOM

SÁNCHEZ MASLOVSKI, FRANCO MARTÍN; GAY, CAROLINA; ECHEVERRIA, SILVINA; HERNÁNDEZ, DAVID; LEIVA, LAURA; FUSCO, LUCIANO

Conferencia; The 2nd International Electronic Conference on Toxins session Mechanism of action and/or pathophysiology of toxins; 2023

Snake venoms are a complex mixture of toxins, mostly of enzymatic nature, such as Phospholipases A2 (PLA2), Metalloproteinases (SVMP), Serine proteases (SVSP), others. At present, there is a growing interest in the search for natural or synthetic inhibitors acting on these components and thus allow mitigating their local and systemic effects. The main damage induced by the venom of B. alternatus is caused by SVMP, for this reason in the present work we proposed the use of 6-o-palmitoyl-l-ascorbic acid (ASC16) as an inhibitor of the toxic effects induced by the SVMP of B. alternatus venom. For this purpose, ASC16 (0.24 mM) was incubated with crude venom (1µg/µL) for 30 min at 37 °C. Proteolytic activity was measured using azocasein as substrate, and tissue injury (edema, hemorrhage and necrosis) was evaluated in a murine model (CF01). ASC16 inhibited 49.6% proteolytic activity, 78.06 % hemorrhagic activity, also the edema produced by the venom in the presence of ASC16 showed significant difference (p