THE FLAVONOID 2’-NITROFLAVONE REDUCES VIABILITY AND MIGRATION IN HUMAN ENDOTHELIAL CELLS.

MIRIAM BENSO; VANINA SOL VACHETTA; LUCIANA SARRIAS; MARIEL MARDER; MARÍA FERNANDA TRONCOSO; MARÍA TERESA ELOLA

Buenos Aires

Congreso; Congreso Anual de Fisiología 2023; 2023

Sociedad Argentina de Fisiología

Introduction: Flavonoids are polyphenolic compounds that cause several antineoplastic effects. We previously described that the synthetic flavonoid 2’-nitroflavone (2’NF) inhibits proliferation and migration of human triple-negative breast cancer (TNBC) cells. Objectives: Our aim pointed to find out 2’NF effects on endothelial cells. Methods: We employed EA.hy926 human endothelial cells to perform viability (MTT) and migration (wound-healing) assays. Two experimental approaches were conducted: A) direct treatments with 2’NF (1.25-40 µM) or vehicle on endothelial cells, and B) endothelial cells treated with conditioned media (CM) derived from TNBC cells pre-incubated with 2’NF (1-25 µM) or vehicle. Results: EA.hy926 cell viability was significantly reduced with 2.5 µM 2’NF (77.98±4.06%; n=5; p0.05) were selected. In scratch assays, in the presence of 1% FBS, direct treatment (A approach) with 1.25 µM 2´NF significantly reduced cell migration (vehicle: 100%; 2’NF: 80.78±3.39%; n=4; p