Contribution of GLAST+ Wnt1+ bone marrow stromal progenitors to the infarcted heart

BORDA M; BUSTOS GR; ALVARADO F; MARTINO GARCET MA; GIARDELLI G; MONTANER A; GONZÁLEZ GE; AQUINO JB

Mar del Plata

Congreso; Annual Meeting SAIC, SAB, AAF, AACYTAL; 2023

SAIC, SAB, AAF, AACYTAL

Objectives: To analyze the contribution of GLAST+ WNT1+ bone marrow stromal progenitors (BMSPs) to the heart after acute myocardial infarction (AMI). Materials and methods: Wnt1Cre;Rosa26tdTomato and GLASTCreERT2;Rosa26tdTomato (tamoxifen-injected at postnatal days 2 or 60) mice were used. AMI was induced by permanent or transient (30 minutes) ligation of a branch of the left coronary artery. After 7 or 30 days, heart samples were sectioned and immunostained. Results: A contribution of GLAST+ WNT1+ BMSPs with neovessels in remote areas of the left ventricle was likely found; however, such a contribution seems transitory. One month after the AMI, Tom+ cardiomyocytes were found in subepicardial areas, adjacent to the area of the lesion. In addition, very few cells morphologically resembling endothelial, sinus node and Purkinje cells were also found within the infarcted area. Conclusions: GLAST+ Wnt1+ BMPs would probably contribute endothelial cells and cells of cardiogenic lineage after AMI. Although the significance of these findings remains to be addressed, they may help the development of new applications for regenerative medicine.