1. Establishment of cell lines and culture of cancer stem cells (CSCS) from veterinary patient-derived tumor samples on lab-on-a-chip (LOC) microdevices

SILVIA GÓMEZ ; ANA PEÑA HERRERA; IMANOL AGÜERO ; MATÍAS TELLADO ; BETIANA LERNER ; MAXIMILIANO PÉREZ ; DENISE BELGOROSKY ; ANA MARIA EIJAN

Congreso; Reunion Anual de Sociedades de Biociencias; 2023

SAIC

Cancer Stem Cells (CSCs) are a minority tumor cell population, associated with treatment resistance, as well as post-surgical recurrences. The identification, quantification and establishment of cell lines derived from primary tumors, can be a useful methodology to predict the evolution of patients and their response to treatment. The use of Lab-On-a-Chip (LOC) microdevices (MD), as a miniaturized support, enables the isolation and monitoring of CSCs. Objective: To develop an assay to study CSCs therapeutic response in LOC, in order to serve as a predictive platform for response to treatment in patients. Methods: Twenty-nine (29) veterinary tumor samples (Sarcomas (SA): 41%, Carcinomas (CA): 31%, Mastocytomas (MA): 17%, Melanomas (ME): 7%, Lymphomas (LY): 3%) were obtained, cultured in 2D and CSCSs were analyzed through sphere culture within LOC MD, using concentrations from 1x105-6x105 cells/ml in low adhesion conditions, DMEM F12 , B27, FGF and EGF in absence of serum. Results: Among of the 29 tumors: 6 were established as 2D cell lines (solid thyroid CA, tubulopapillary CA, bladder CA, low and high grade MA); 5 samples were grown as spheres (% Sphere formation efficiency (SFE±SD) : SA: 0.37±0.2, Bladder CA:0.28±0.05, Thyroid CA: 0.37±0.3, Squamous cell CA: 0.1±0.02 and LY: 0.19±0.1 ). In a thyroid CA tumor sample, cell viability (MTS) was measured under carboplatin treatment, determining an inhibitory dose of 57 µM (p