EFFECT OF INTRACISTERNAL RAD-IGF1 IN ANXIETY-LIKE BEHAVIOR IN A RAT MODEL OF NEUROTOXICITY

CHAMPARINI, LEANDRO GABRIEL; HERRERA, MACARENA LORENA; JÁVEGA COMETTO, MATÍAS; HEREÑÚ, CLAUDIA BEATRIZ

San Luis

Congreso; SAN 2023 Annual Meeting; 2023

Sociedad Argentina de Neurociencias

Parkinson’s disease is a neurodegenerative disorder characterized by the progressiveloss of dopaminergic neurons, resulting in a range of motor and non-motor symptoms.Although motor deficits are the main criteria for diagnosis, there is growing evidencethat non-motor symptoms may also play an important role in the disease progression.We aimed to investigate the possible effect of IGF-1 in the progression of emotional-likebehavior deficits in a rat model of neurotoxicity inducedby 6-OHDA infusion into thedorsolateral striatum. We assessed the behavioral changes in male Wistar rats after 1, 2and 3 weeks post-lesion using the elevated plus maze, light-dark box, and forcedswimming tests. After 6-OHDA infusion, immunohistochemical analysis revealed adecrease in tyrosine hydroxylase immunoreactivity in the substantia nigra, ventraltegmental area, and striatum, indicating partial lesion of the nigrostriatal dopaminergicsystem. Furthermore, we found an increase in mRNA levels of proinflammatorycytokines IL-1 in the striatum. Our results showed anxiety-like behavior at 2 and 3 weekspost-lesion, beforethe onset of motor impairment. And IGF-1 was able to prevent suchbehavior at week 3.