CYTOKINES PROFILE INDUCED IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF PIG AFTER STIMULATION IN VITRO WITH DIFFERENT CPG-ODN

GIULIANA VEDELAGO; LUZ MARÍA PALACIOS; FEDERICO RUIZ MORENO; CONSTANZA MARÍN; SANTIAGO PALMA; GABRIEL MORÓN; FABRICIO ALUSTIZA; MARÍA INÉS CRESPO; BELKYS MALETTO

San Luis

Congreso; LXXI Reunión Científica Anual de la Sociedad Argentina de Inmunología SAI; 2023

Sociedad Argentina de Inmunología SAI

We developed an innovative strategy for formulating vaccine components, whichinvolves the formulation of antigen and CpG-ODN with a nanostructure formedby self-assembly of 6-O-ascorbyl palmitate (Coa-ASC16). Previously, wedemonstrated that mice immunized with the OVA/CpG-ODN/Coa-ASC16nanoformulation elicited antigen-specific antibody and cellular responsessuperior to those induced by an aqueous solution of OVA and CpG-ODN. CpGODNs, due to their chemical structures and immunostimulatory effects, can becategorized into three classes: A, B, and C. Furthermore, the immunostimulatoryactivity triggered by CpG-ODNs varies among different species. Our goal is tovalidate the CpG-ODN/Coa-ASC16 adjuvant strategy in the porcine species. Toidentify which CpG-ODN elicits the best response in pigs, peripheral blood fromLarge White x Landrace pigs was collected from the jugular vein in vacutainertubes containing 18 mg of EDTA K2. Peripheral blood mononuclear cells (PBMC)were isolated through FICOLL gradient separation and incubated at 37°C in anenvironment with 5% CO2 for 12 hours with four different types of CpG-ODN:2395, 1826, 1018, and 2007. Medium without CpG-ODN was employed as thecontrol (baseline). The mRNA expression of cytokines and TLR9 was assessedthrough RT-qPCR. Our findings indicate that CpG-ODN 2395 (a class C CpGODN originally used for humans and mice) is capable of inducing a broadspectrum of innate immune response mediators, including IL-6, IL-12p40, TNFα,IFNβ, and IFNγ (p