EXPRESSION OF TYPE III INTERFERONS AND ITS RELATIONSHIP WITH TYPE I AND II INTERFERONS IN THE DUODENAL MUCOSA OF CELIAC PATIENTS.

IRIBARREN, MARÍA LUZ; PEREZ, FEDERICO; DUCCA, GERÓNIMO; CAROLINA N. RUERA; GUZMAN LUCIANA; MENENDEZ, LORENA; GARBI, LAURA; EUGENIO ANTONIO CARRERA SILVA; CHIRDO FERNANDO

San Luis

Congreso; LXXI Reunión anual de la sociedad argentina de inmunología; 2023

Sociedad argentina de inmunología (SAI)

EXPRESSION OF TYPE III INTERFERONS AND ITS RELATIONSHIP WITH TYPE I AND II INTERFERONS IN THE DUODENAL MUCOSA OF CELIAC PATIENTS.María Luz Iribarren1#, Federico Perez1#, Gerónimo Ducca1 , Carolina N. Ruera1 , Luciana Guzman2, Lorena Menendez2 , Laura Garbi3 , Antonio Carrera Silva4 , Fernando G. Chirdo1Instituto de Estudios Inmunológicos y Fisiopatológicos-IIFP (UNLP-CONICET). Servicios deGastroenterología de 2Hospital de Niños and 3Hospital San Martin. La Plata. 4Instituto de Medicina Experimental-IMEX (CONICET).# These authors equally contributed to this work.Objective: Celiac Disease (CD) is a chronic inflammatory disease caused by anabnormal T cell-mediated immune response of genetical susceptible individualsto dietary gluten.Interferons (IFNs) are divided into three families (type I, type II, and type III) onthe basis of sequence homology. While type I and II IFNs have been associatedwith CD, information about type III IFNs is limited. The aim of this work was toevaluate the expression of type III interferons (IFNL1 and IFNL2/3) in healthyhuman small intestine and samples from CD patients, and its relationship withboth type I and II IFNs.Material-Methods: Duodenal biopsies were collected from untreated CDpatients (CD) and non-celiac (NC) individuals and analyzed by RT-qPCR andfluorescent microscopy. Ethics committees from Health Institutions approved thisprotocol.HT-29 cells (model for intestinal epithelial cells) were cultured in 24 wells platefor 72 or 24 h after incubation with 50 ng/ml IFN and 1000 U/ml IFNβ. Samplesfrom cell culture were used for RT-qPCR analysis.Results: Since type I and II IFNs have been associated with CD, we decided toanalyse their transcripts levels as well as some of their target genes in duodenalsamples. A significant upregulation of IFNβ1 (p-value < 0.05), IFN (p-value