THE SEROPREVALENCE OF MYCOBACTERIUM AVIUM SUBSP PARATUBERCULOSIS IN TWO BEEF CATTLE OF BUENOS AIRES ARGENTINA.

COLAVECCHIA S.; FERNÁNDEZ B.; TROPEANO M; FERNANDO PAOLICCHI; MUNDO SL.

Riviera Maya.

Simposio; 14th International Colloquium on Paratuberculosis.; 2018

International Colloquium on Paratuberculosis.

Paratuberculosis (PTB) is a gastrointestinal contagious chronic disease that affects ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Although control programs based on these principles have reduced prevalence of MAP infection in dairy herds, they have generally not eliminated the infection. Prevalence in most regions is currently unknown, In Argentina there are no updated prevalence data, it is estimated between 8 and 20%, and there is no more information because tuberculosis control measures are still being implemented.The aim of this work is to estimate the seroprevalence of bovine PTB in Argentina.We focused on cattle farms located in Buenos Aires province, which is a major region for beef cattle production257serum samples from 2 herds of beef farms in Buenos Aires province where PTB has been proven by culture. Sampling was carried out at random and calculating the number of sera with an expected prevalence of 15% and a confidence interval of 95%: Laprida (n=169/4588) and Chacabuco (n=46/59).We developed an in-house ELISA using PPA (Allied monitor) as antigen and anti- IgG bovine HRP (KPL) as second antibody. Positive and negative serum samples were used as controls (Allied monitor).The seroprevalence obtained were 4.3% (Chacabuco) and 5.9% (Laprida). Animals were cathegorized and percentage of positive samples were calculated: heifers (10.3%), bulls (3.0%), empty cows (5.0%), pregnant cows (5.5%) and none calves were detected as positive. The positive results shows that it may be vary among animal cathegory as was described previously. The percentage of seroprevalence obtained for the breeding herds (between 4.3 and 5.9) shows a difference with the previous data, this could be due to the different management carried out in dairy farms in comparison with the extensive breeding in the province of Buenos Aires.Then, the seroprevalence here evaluated may change the number of animals to be studied for complete the survey in Argentina.