EVALUATION OF THE METABOLIC PROFILE IN BIOLOGICAL SAMPLES OF HEALTHY AND ALZHEIMER’S DISEASE PATIENTS IN THE CONTEXT OF A MEDITERRANEAN DIET

MOSSE JUANA INÉS; YUSTE SILVIA; IÑIGUEZ MARIA; MATUTE TOBIAS BELINDA; LOPEZ ALAVA SARA; PEREZ MATUTE PATRICIA; MOTILVA MARÍA JOSÉ

Toledo

Congreso; Lifestyle, diet, wine and health; 2023

FIVIN

The Mediterranean Diet has been proposed as a dietary pattern model toprevent or delay brain dysfunction in part associated to the polyphenol-rich foodintake including red wine. Studyingthe metabolic profile of Alzheimer’s disease (AD) patients versus healthy (C)volunteers may offer the possibility to identify potential targets to treat ordelay the onset of the disease. The objective of this work was to identify differences in the urinal, plasmaticand fecal metabolic profile in AD patients compared with matched controls inthe context of a Mediterranean Diet with special focus on wine consumption. Forthis, C (n=53) and AD patients (n=50) of both sexes (55-75 y) recruitedat the Hospital San Pedro of La Rioja, Spain,participated in an observational study. Data concerning dietary habits wereobtained during a personal interview. Phenolic metabolites were determined inurine and feces by ultra-high performance liquid chromatography coupled to massspectrometry (UPLC-MS/MS) whereas plasmatic and fecal short chain fatty acids includingacetic, propionic, butyric, isobutyric, valeric, isovaleric and caproic acidswere analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Amajor proportion of wine consumers was observed in the C group (70%) than inthe AD group (44%), these were categorized into low (43 and 36% in C and AD,respectively), moderate (43 and 50% in C and AD, respectively) and highconsumers (14% in both C and AD). Metabolomic analysis revealed major contentof phenolic species (p<0.05) associated with wine consumption includingmalvidin, hydroxytyrosol, phenyl acids, resveratrol and gallic acid related compoundsin the urine of the C than the AD group. Feces of healthy participants showedhigher abundance of pyrogallol and some phenolic acids (p<0.05) and tendedto contain more caproic acid (p=0.0718) compared to fecal samples of AD volunteers.In plasma, butyric acid tended to be higher in the control group (p=0.088). Thesepreliminary data suggest differences in the metabolic profile of biologicalsamples (urine, plasma and feces) between healthy and AD patients which couldbe considered as interesting candidates to study their biological role in theprevention of cognitive diseases