INVOLVEMENT OF TNF-ALPHA; IN THE ACTIVATION OF GAMMA DELTA T LYMPHOCYTES BY HUMAN GLOMERULAR ENDOTHELIAL CELLS EXPOSED TO SHIGA TYPE 2 TOXIN. ROLE IN THE PATHOGENESIS OF HEMOLYTIC UREMIC SYNDROME

ROSSO, DAVID A; ROSATO, MICAELA; GOMEZ, FERNANDO D; ALVAREZ, ROMINA S; SHIROMIZU, CAROLINA M; IBARRA, CRISTINA; AMARAL, MARÍA MARTA; JANCIC, CAROLINA C

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC, SAI, AAFE y NANOMED-AR

The hemolytic uremic syndrome (HUS) associated with diarrhea, a consequence of Shiga toxin (Stx)-producing Escherichia coli infection, is a common cause of pediatric acute and chronic renal failure. Stx type 1 and type 2 (Stx1 and Stx2) produced by those bacteria are the main factors related with HUS that trigger kidney damage. Stx2-producing strains are associated with severe cases of HUS in Argentina. gd T cells are a specialized subset of T lymphocytes, which act as early sensors of cellular stress and infection. They can exert cytotoxicity against infected and transformed cells and produce cytokines and chemokines. In this work, we studied the activation of human peripheral gd T cells in response to human glomerular endothelial cells (HGEC) exposed to Stx2 (0.01 ng/ml, 24 h) or their conditioned medium. We analyzed CD69, CD107a, and perforin expression by flow cytometry; and cytokine production by ELISA and intracellular staining in gd T cells after incubation with Stx2-treated HGEC or their conditioned medium. We evaluated by confocal microscopy, the interaction between gd T cells and HGEC treated or not with Stx2 and perforin distribution. As result, we observed an increase in TNF-α, IFN-γ, and cell interactions (p