Implications of TGF-β in the early progression of breast cancer

SCIACCA M ; ZAMBRANO M ; MARINO L ; EIJAN A M; LODILLINSKY C

Simposio; BUENOS AIRES BREAST CANCER SYMPOSIUM 2021; 2021

Implications of TGF-β in the early progression of breast cancerAUTHORSSciacca M1, Zambrano M1, Marino L2, Eiján AM1 and Lodillinsky C1. 1.Research Area, Instituto de Oncología Ángel H. Roffo, Universidad de Buenos Aires, Buenos Aires, Argentina2. Pathology Department, Instituto de Oncología Ángel H. Roffo, Universidad de Buenos Aires, Buenos Aires, ArgentinaThe TGF-β pathway activates the EMT process and enhances the stemness of normal and cancerous breast cells. These suggests that TGF-β would be implicated in the early stages of breast cancer progression. On this basis, we propose to understand the implication of TGF-β in the dialogue between the two populations that conformed the mammary duct: luminal cells (LEP) and myoepithelial cells (MEP).The cellular mouse model LM38 is composed of LM38-LP (MEP and LEP), LM38-HP (LEP) and LM38-D2 (MEP). Previously, we showed that only LM38-LP was able to develop DCIS tumors after intraductal injections, suggesting that bi-cellular interaction could confer an advantage for tumor formation and progression. Moreover, treatment with conditioned medium of MEP induced viability on LM38-LP.LM38-D2 showed higher levels of TGF-β1 mRNA than LM38-LP (qPCR, p