-Nanotechnology in Oncology :The use of microfluidic device to analyse bladder cancer stem cells

FERNÁNDEZ-CABADA TAMARA, ; AGÜERO, EDUARDO IMANOL, ; LANGLE YANINA, ; PEÑAHERRERA-PAZMIÑO ANA BELÉN, ; BHANSALI SHEKHAR,; BOOTH ROSS , ; PÉREZ MAXIMILIANO, ; LERNER BETIANA, ; EIJAN ANA MARIA.

MAR DEL PLATA

Congreso; Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS).; 2018

Introduction: Lab on a Chip (LOC) culture systems are presented as a useful tool that allows thegrowth of tumor cells, their identification and isolation using specific markers. This type of devicehas the additional advantage of using low amounts of cell and reagents. Cancer stem cells(CSCs), a small heterogeneous population of cancer cells, associated with metastases andtumor recurrences. They can be identified by their growth independent of anchorage and bymarkers such as Oct4 and CD44, among others. The objective of this study was to analyze in aLOC device, using an invasive bladder cancer cell line (MB49-I), the growth of spheres, toidentify CD44 expression and to capture differential CSC through functionalization of LOCsurface with CD44 antibody. Results: Plating increasing number of cells (8, 16 and 32cell/μl) wedetermined 32cell/μl as the optimal number of cells to seed, resulting in a 70% of sphereforming efficiency after 14 days. The resulted spheres expressed CD44, observed directly insideof the microdevice (immunofluorescence). Furthermore, the surface of the microdevice wassuccessfully functionalized with CD44 antibody using silane, enabling to capture CSC and toidentify cells by this specific surface marker. In addition, we observed that the volume ofreagents used in the microdevice resulted in 29 times lower compared to traditional plates ofcell culture. Conclusion: LOC devices are a promising tools since using a minimum amount ofreagents and samples, allow growth, identification and isolation of cancer cells. Thismicrodevice promises to be considered as a novel technology to be used as a complement orreplacement of traditional studies in the culture of tumors and could be an alternative in thefuture to isolate circulating CSCs