Study of silybin flavonoid capability to enhance antitumor effect of radiotherapy on MB49 murine bladder cancer model.

BÁRBARA PRACK MC CORMICK ; ANA MARIA EIJÁN ; EDUARDO OMAR SANDES

San Pablo

Workshop; Course ? Advances in molecular oncology: translating molecular biology into cancer treatment-. 2nd to 8th february 2013, Sao Paulo, Brazil.; 2013

Fundação de Amparo à Pesquisa do Estado de São Paulo

Study of Silybin flavonoid capability to enhance antitumor effect of Radiotherapy on MB49 murine bladder cancer cells. Bárbara Prack Mc Cormick, Ana Maria Eiján, Eduardo Omar Sandes. Instituto de Oncología Ángel H. Roffo. Avenida San Martin 5481, CABA, Argentina. Radiotherapy (RT) is widely used in tumor treatment, but tumor cells sometimes acquire resistance resulting in recurrence and failure in many patients. The transcription factor NF-kB activates survival pathways in response to RT, proposing it as a therapeutic target to improve RT effect. Natural compounds of the flavonoid family have the ability to block NF-kB pathway. Therefore, we analyzed Silybin (Sb) flavonoid capability to enhance antitumor effect of RT on MB49 murine cells in vitro and in vivo. In vitro, RT (LD50 at 120h post-RT: over than 8Gy) and Silybin (LD50: 120μM) induced dose-dependent cytotoxicity; Silybin magnified RT inhibitory effect on cell viability (Viability. RT: 89%, Sb: 64%, RT+Sb: 31%, p=0.01) and clonogenic capacity (2Gy vs. 2Gy+Sb80μM p