EVALUATION OF THE ANTIFUNGAL ACTIVITY OF EXTRACTS FROM DALEA PAZENSIS RUSBY

NEGRO MELISA FABIANA; EINY NALLYBE BEDOYA AGUIRRE; ORTEGA MARIA GABRIELA; PERALTA MARIANA ANDREA

Minas Gerais

Congreso; II CONGRESO INTERNACIONAL DE CIENCIA, BIODIVERSIDAD Y SUSTAINABILIDAD; 2023

Universidade Federal de Minas Gerais UFMG Campus Pampulha

Dalea pazensis Rusby is an endemic species from Bolivia. Previous studies determinedthe presence of prenylated flavonoids in the benzene extract of its roots. It has beenreported that this type of compound has antifungal activity. Candida albicans is a yeastcapable of producing superficial and systemic mycoses called candidiasis. Multiple drugresistance (MDR) is the main mechanism responsible for resistance in clinical strains ofCandida. The objective of this study was to evaluate the antifungal activity of risingpolarity extracts obtained from the roots of D. pazensis, by determining the minimuminhibitory concentration (MIC) against clinical resistant strains of C. albicans. D.pazensis was collected in the town of Yotala, Department of Chuquisaca, Bolivia. Fourextracts with organic solvents of increasing polarity were obtained from the dried roots:hexane, chloroform, ethyl acetate, and ethanol. Soxhlet was used as the extractionmethodology. The antifungal activity of the extracts was evaluated by microdilution in a96-well plate (according to CLSI standards), on two clinical strains of C. albicans, oneresistant (CaR) and the other sensitive (CaS) to azoles. The optical density was measuredat 540 nm in the presence of the four extracts. Those that were active at 500 μg/ml werethen evaluated at the following concentrations: 31, 64, 125, and 250 μg/ml to determinethe MIC. The chloroform extract turned out to be the most active, presenting the MIC at125 μg/ml, with percentages of inhibition of CaS and CaR growth of 58.22% and 69.65%,respectively. Likewise, at 250 μg/ml, this extract inhibited the growth of both strains bymore than 90%. For the hexane and ethyl acetate extracts, the MIC values were 250μg/ml, inhibiting 70% and 50% respectively, against CaS and Car both strains. Theethanolic extract was not active at 500 μg/ml. According to these results, the medicinalpotential of the extracts of D. pazensis was evidenced, against clinical strains of C.albicans sensitive and resistant to azoles. It is proposed to deepen the phytochemical andpharmacological investigations of the most active extracts from D. pazensis roots.