PRENATAL ANDROGENIZATION AFFECTS OVARIAN LOCAL FACTORS THAT NEGATIVELY IMPACT ON FOLLICULAR DEVELOPMENT

MOTTA, ALICIA BEATRIZ; ABRUZZESE, GISELLE ADRIANA; FERRER, MARIA JOSE

Mar del Plata

Congreso; Reunión Conjunta de Sociedades de Biociencias 2022; 2022

SAIC

Prenatal androgen excess contributes to Polycystic Ovary Syndrome (PCOS) development. PCOS is the main cause of oligo-anovulation at reproductive age. Ovarian factors influence folliculogenesis, but it is still unknown how androgen excess affects them, impacting follicular growth and fertility. Thus, we aimed to study the effect of prenatal androgenization on local ovarian factors involved infolliculogenesis.Pregnant Sprague Dawley rats were injected with testosterone (Prenatal androgenized group, PA) orvehicle (Control group) from days 16 to 19 of pregnancy. The female offspring were characterizedaccording to the estrous cycle, and we obtained: regular ovulatory Control animals, and irregularovulatory (PAiov) and anovulatory (PAanov) animals in the PA group. At 90 days of age, we analyzed the hormonal profile and ovarian parameters by qPCR and Western Blot.The analyzed proliferation markers were altered in the PA group. We found decreasedEstradiol2/Testosterone ratio and Sf1 mRNA levels in the PA group (p