Metabolic and cardiovascular alterations induced in rats by high fat and fructose diets during growth can be attenuated by zinc supplementation

MEDINA AGUSTINA; PAEZ DIAMELA TATIANA; POLERO FRANCO; JUAN MANUEL GAETTANI; GABRIELA NOCETT; MORIONDO MARISA MABE; DOMÍNGUEZ JUANA; CAROLINA OLANO; VALERIA ZAGO; ROSANA ELESGARAY; CAROLINA CANIFFI; CRISTINA ARRANZ; ANALIA TOMAT

La Plata

Congreso; 2022 Annual Meeting of International Society for Heart Research. Latinoamerican Section (ISHR LAT); 2022

Introduction: High fat high fructose diet induces metabolic disorders in adult life. In previous studies we demonstrated that zinc has antioxidant properties and can regulate metabolism. Objective: To evaluate the effects of postnatal zinc supplementation on systolic blood pressure (SBP), retroperitoneal adipose tissue (RPAT) and intermediate metabolism in adult male Wistar rats fed a high fat and fructose diet during growth. Methodology: Male wistar rats fed since weaning (21 days) to adult life (81 days): Cc: zinc control diet (30 ppm); Cgf: zinc control-high fat diet and fructose 10% in drinking water; Zgf: zinc supplemented (190 ppm)-high fat diet and fructose 10% in drinking water. At day 81, we determined SBP, metabolic profile, RPAT morphology and blood oxidative stress. N=5 for each group. Two-way Anova, test post-hoc: Bonferroni. Results: Cgf showed higher body (Cc: 456±15; Cgf: 515±10*; Zgf: 476±10 g) and RPAT (Cc:1,4±0,1; Cgf:3,1±0,4***; Zgf:2,7±0,1g)weights; glycemia, triglyceridemia (Cc: 115±8; Cgf: 156±8**; Zgf: 146±8 mg/dl), uricemia levels and plasma lipid peroxidation (TBARS) after 6 hours fast; SBP and adipocyte area (Cc: 4705±193; Cgf: 7450±838**; Zgf: 7435±484 μm2 ) compared to Cc. Zgf rats exhibited lower values of SBP (Cc:133±2; Cgf: 159±2*;Zgf: 144±1$$ mmHg), plasmatic lipid peroxidation (Cc:3,1±0,2; Cgf:12,1±1,6***; Zgf:2,5±0,7$$$ nmol TBARS/ml), basal glycemia (Cc: 119±4,Cgf: 140±5**,Zgf: 125±2$$ mg/dl) and uricemia (Cc: 0,9±0,1; Cgf:1,9 ± 0,3 *; Zgf:1,0±0,1$$ mg/dl) than Cgf. Conclusion: High fat and high fructose diet during growth induces cardiovascular and metabolic alterations in adult life. These alterations could be attenuated by zinc supplementation due to its antioxidant properties and the multiple functions in glucose metabolism