TARGETTING OF MITOCHONDRIAL PEPTIDE HUMANIN TO IMPROVE CHEMOSENSITIVITY IN GLIOBLASTOMA CELLS

ASAD, ANTONELA S.; NICOLA CANDIA, ALEJANDRO JAVIER; MARCHESINI, ABRIL; GONZALEZ, NAZARENO; CANDOLFI, MARIANELA; PIDRE, MATÍAS LUIS; PÉREZ KUPER, MELANIE; SAGRIPANTI SOFÍA; VERA, MARIANA B.; SEILICOVICH, ADRIANA; PEÑA AGUDELO, JORGE A.; GARCIA FALLIT, MATÍAS; GLIENKE, LEILANE; AMORÓS MORALES, LESLIE C.; VIDELA-RICHARDSON, GUILLERMO A.

Mar del Plata

Congreso; Reunión de Sociedades de Biociencias 2022 (SAIC, SAI, SAFis); 2022

SAIC

Humanin (HN) is amitochondrial peptide with a robust cytoprotective many cell types.HN can interact with proteins of the bcl-2 family or be released andbind with two membrane receptors: a trimetric receptor, and the FPR-2receptor. HN protects normal tissues from chemotherapy, and theadministration of HN analogs has been proposed as a therapeuticapproach for degenerative diseases. However, its role on thepathogenesis of cancer is poorly understood. Here we aimed toevaluate whether HN affects chemo-resistance of glioblastoma (GBM)cells. We first assessed the effect of chemotherapy on HN expressionin murine (GL26) and human (U251) GBM cell lines, as well as inprimary cultures from GBM biopsies. By immunofluorescence we observedthat cisplatin upregulates HN in all the cells evaluated. To analyzethe effect of HN on chemotherapeutic cytotoxicity, we used a HNanalog peptide (HNG). In human GBM cells we observed that HNGabolished the cytotoxic and antiproliferative effect of cisplatin,restoring viability and clonogenic capacity (Two-way ANOVA p