INVESTIGADORES
NUÑEZ Nicolas Gonzalo
congresos y reuniones científicas
Título:
Soluble factors released by DU145 human prostate cancer cells stimulated with Poly I:C-liposomes promote the activation of monocyte-derived dendritic cells.
Autor/es:
GATTI, GERARDO; NÚÑEZ, NICOLÁS; GIRAUDO, CONSTANCIO; MACCIONI, MARIANA
Lugar:
Buenos Aires
Reunión:
Congreso; First Franco-Argentine Scienctific Meeting in Immunology; 2010
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Tumors derived factors significantly inhibit the generation as well as the activation of dendritic cells (DCs).
Polyinosinic-polycytidylic acid (Poly I:C, PIC), a dsRNA analog, can trigger the secretion of type I Interferon and
induce apoptosis in tumor cells via the innate recognition receptors melanoma-differentiation-associated gene
(MDA)-5 and retinod-inducible gene (RIG)-I in the cytosol or via Toll-like receptors 3 in intracellular endosomes.
Thus, we hypothesized that prostate tumor cells activated via these receptors could release soluble factors that
can restore the suppressive effect of tumor derived factors on DCs. In order to see if factors secreted by PICstimulated
DU145 cells could somehow overcome this already described inhibitory effect, DU145 cells were
cultured with PIC-liposome complex (0.1 wg/ml) for 4 hours, washed three times with PBS, incubated for 20
additional hours and the culture supernatant was then harvested and filtered (CM-PIC). As control, non
stimulated DU145 cell culture supernatant was also collected (CM). Human monocyte-derived DCs (MoDCs)
cells were matured with LPS in the presence of CM-PIC or CM. MoDCs stimulated with LPS in presence of CMPIC
increased the expression costimulatory molecules compared to MoDC stimulated with LPS in presence of
CM (CD86 MFI: 178 vs 97; CD80 MFI: 35 vs 26; CD40, MFI: 23 vs 14). Furthermore, the levels of IL12p70 were
significantly increased in LPS-stimulated MoCDs incubated with CM-PIC at 24h (670.1±2.9 vs 90.3±16.3 pg/ml),
and at 48h (926.5±14.2 vs 127.5±1.5 pg/ml), p