METABOLIC PROFILE OF MATURED BOVINE OOCYTES VITRIFIED WITH CRYOTECH®

ANTONELLA DONATO; PABLO CETICA; GABRIEL DALVIT; SERGIO MORADO

Jornada; XVIII Jornadas de la Sociedad Argentina de Biología; 2016

The objective was to evaluate reactive oxygen species (ROS) production and redox state in vitrified matured bovine oocytes. Cumulus-oocyte complexes were obtained by aspiration of antral ovarian follicles and matured in 199 medium with bovine fetal serum, FSH and LH at 39°C, 5% CO2 in humidified air for 22h. Matured oocytes were denuded and a group was vitrified through the minimum volume vitrification method Cryotech®. Then vitrified oocytes were subjected to warning. Later we measured the autofluorescence intensity of NAD(P)H at three different times (0, 3, 21h; n= 180 oocytes). To evaluate ROS production we incubated bovine oocytes (n= 180 oocytes) in 2?-7?-dichloro-dihydro-diacetate fluorescein (DCHFDA), determining the ratio between DCHFDA fluorescence and average diacetate fluorescein brightness. As regards the oocyte redox state the control group presented a significant increase at 21 compared with 0 and 3h (p