OXIDATIVE AND MITOCHONDRIAL ACTIVITIES IN VITRIFIED PORCINE OOCYTES

AILÉN APARICIO; DANIELA PINCHETTI; PABLO CETICA; GABRIEL DALVIT; SERGIO MORADO

Jornada; XVIII Jornadas de la Sociedad Argentina de Biología; 2016

To date, porcine oocyte vitrification presents suboptimal results, which could be due to changes in their redox state. Therefore, the aim of this study was to evaluate oxidative and mitochondrial activities on in vitro matured porcine oocytes after vitrification-warming. Cumulus-oocyte complexes were collected by aspiration of antral follicles and matured in medium 199 + porcine follicular fluid + gentamicin sulfate + FSH + LH under mineral oil at 39ºC for 48h in 5% CO2 atmosphere. Then, oocytes were denuded and vitrified by Cryotech© method. Matured non-vitrified porcine oocytes were used as a control group. Oxidative and mitochondrial activities were determined by RedoxSensor Red CC-1/MitoTracker Green FM dual stain at 0, 3 and 21h after warming. For Mitotracker Green FM stain, both vitrified and fresh mature oocytes showed a decrease in luminosity at 21h (p