VARIACIONES EN LA TOMA DE MUESTRA DESTINADA A LA EVALUACIÓN

SARLO,G;; MEDICI,S; PORRINI,D; EGUARAS,M

Punta del Este

Congreso; 1º Congreso de Apicultura del Mercosur; 2005

Nosema apis, a microsporidian pathogen of adult Apis mellifera, is worlidwide distributed (Fries, 1984). Different methodologies are proposed to determine the level of infection caused by Nosema in honeybee and they all have varied degrees of certainty (Fries, y Ekborn, 1984; Malone et al, 1993). The objective of the present work is to determine if there are significant differences in the spore count when the sample is collected from the outside of the hive or when it is taken from the honey combs placed in the inside. The results shows that there are significant differences (p<0.01) between the samples collected from the inner side of the hive and those taken from the outside, being the spores counts higher in those who were obtained from the exterior of the hive. We conclude that the sampling method that better represents the level of infection caused by Nosema apis is those where the bees are obtained when they are coming back from the foraging. This information will allow us to establish more precise strategies of control and to include this sampling method in the current national protocols of hygiene and quality, with the posibility of obtaining comparable results all over the country and with the only objective of detect, quantify and control this disease that causes son many adverse effects in the hives and the honey yield., a microsporidian pathogen of adult Apis mellifera, is worlidwide distributed (Fries, 1984). Different methodologies are proposed to determine the level of infection caused by Nosema in honeybee and they all have varied degrees of certainty (Fries, y Ekborn, 1984; Malone et al, 1993). The objective of the present work is to determine if there are significant differences in the spore count when the sample is collected from the outside of the hive or when it is taken from the honey combs placed in the inside. The results shows that there are significant differences (p<0.01) between the samples collected from the inner side of the hive and those taken from the outside, being the spores counts higher in those who were obtained from the exterior of the hive. We conclude that the sampling method that better represents the level of infection caused by Nosema apis is those where the bees are obtained when they are coming back from the foraging. This information will allow us to establish more precise strategies of control and to include this sampling method in the current national protocols of hygiene and quality, with the posibility of obtaining comparable results all over the country and with the only objective of detect, quantify and control this disease that causes son many adverse effects in the hives and the honey yield.et al, 1993). The objective of the present work is to determine if there are significant differences in the spore count when the sample is collected from the outside of the hive or when it is taken from the honey combs placed in the inside. The results shows that there are significant differences (p<0.01) between the samples collected from the inner side of the hive and those taken from the outside, being the spores counts higher in those who were obtained from the exterior of the hive. We conclude that the sampling method that better represents the level of infection caused by Nosema apis is those where the bees are obtained when they are coming back from the foraging. This information will allow us to establish more precise strategies of control and to include this sampling method in the current national protocols of hygiene and quality, with the posibility of obtaining comparable results all over the country and with the only objective of detect, quantify and control this disease that causes son many adverse effects in the hives and the honey yield.