Decreased protein expression and activity of intestinal multidrug resistance-associated protein 2 (Mrp2) in a high fat diet model: potential role of oxidative stress (OS).

ZECCHINATI, FELIPE; BARRANCO, MARÍA M.; RICARDI, LAURA; ARANA, MAITE R.; PERDOMO, VIRGINIA; LUQUITA, MARCELO G.; MOTTINO, ALDO D.; GARCÍA, FABIANA; VILLANUEVA, SILVINA S. M.

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Consumption of a high fat diet is a key factor in the development of pathophysiological alterations leading to metabolic disorders such as insulin resistance and obesity. Mrp2, an ABC transporter acting as intestinal biochemical barrier, strongly influence the bioavailability, and hence the efficacy and safety, of orally administered drugs. Interestingly, several of these drugs are prescribed to patients with such alterations. We here evaluated the effect of standard diet enriched with 40% kcal from bovine fat (HFD) to C57BL/6J male mice for 8 weeks on jejunal Mrp2 expression, by western blot, and on its transport activity, by using the everted intestinal sacs model. Control mice (C) received standard diet. Fat consumption led to high plasma triglycerides and glycemia levels, as well as insulin resistance and a marked increase of epididimal fat in HFD vs C (p