Adipose tissue and insulin-resistance. Beneficial effects of glp-1 agonists

QUINTANILLA, MARÍA FLORENCIA; TOUCEDA, VANESSA; BARCHUK, MAGALÍ; FINOCCHIETTO, PAOLA; MORALES, CELINA; LOPEZ, GRACIELA; FRIEDMAN, SILVIA; SCHREIER, LAURA; BERG, GABRIELA; MIKSZTOWICZ, VERÓNICA

Mar del Plata

Encuentro; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2022

Sociedad Argentina de Investigación Clínica

Insulin-resistance (IR) is characterized by adipose tissue (AT) expansion associated with extracellular matrix (ECM) remodeling. Metalloproteinases (MMPs) are endopeptidases involved in adipogenesis and angiogenesis, and they are proposed as pharmacological targets. Liraglutide (L), a glucagon-like peptide type 1 agonist, has emerged for the management of IR, and its effects on AT are still investigated. Aim: to evaluate the effect of L on MMPs activity in an animal model of IR. Methods: male Wistar rats (180-200 g) were divided in: Control (C, n=11) fed with standard diet, and sucrose rich diet group (SRD, n=14) fed with standard diet and sucrose 30% in drinking water during 15 weeks. Then, both groups were subdivided according to subcutaneous administration of L (0.6 mg/kg/ day) for 5 weeks. The study was approved by the Ethic Committee- FFYB (UBA). Serum glucose, and lipid and lipoprotein profile were measured. Visceral AT (perirrenal, intestinal and epididymal) was removed and weighed. In epididymal AT (EAT) histological characteristics, MMPs activity by gelatinolytic zymography and antioxidants enzymes (SOD and Catalase) were evaluated. Results: as expected, SRD presented higher visceral AT mass (p