Producción de lipasas por hongos endófitos del género Umbelopsis y Ophiostoma aislados de la madera de Nothofagus

AQUINO, M.D.; SAPARRAT, M.C.N.; MOLINA, M.; PILDAIN, M.B.

Encuentro; Tercer Encuentro & Primer Workchop de la Red Argentina de Tecnología Enzimática; 2021

Many industrial applications of lipases have stimulated interest in searching from microbiologicalsources and significant efforts have been concentrated to improve production and optimize yield.The bioprospecting of enzyme-producing microorganisms leads to the exploration of different taxes and environments. In this sense, the Patagonian endophytic fungi are a group of organisms with great potential for the production of new metabolites. Some species of the fungal genera Ophiostoma and Umbelopsis have been reported as lipase producers. Given the high frequency of appearance of representatives of these genera in the Patagonian Andean forests, this work aimed to evaluate the ability to synthesize lipases from isolates of 3 species of Umbelopsis and 5 of Ophiostoma. Their ability to synthesize hydrolytic enzymes in a plate using Tween 20 as a substrate was tested and the activity was estimated semi-quantitatively as the ratio of hydrolytic halo to mycelium diameter. The activity was measured using 1,2-o-dilauryl-rac-glycero-3-glutaric acid- (6-methylresofurin) -ester as substrate. In the plate assays, the highest levels were detected in the CIEFAPcc 475 O. piliferum isolate (3.17 U/E), while for the Umbelopsis genus the highest was detected for the U. vinacea isolate (2.07 U/E). These species have already been reported as lipase producers, but there are no activity records for isolates from Patagonia. These results serve as the basis for optimizing the production of its extracellular lipases depending on the type of substrate used and the conditions of the fungus culture and the enzymatic reaction.