LEPTIN REGULATES THE EXPRESSION OF GENES RELATED TO LIPID STORAGE IN SERTOLI CELLS

MARINA ERCILIA DASSO; GUSTAVO MARCELO RINDONE; AGOSTINA GORGA; CECILIA LUCIA CENTOLA; ELIANA HERMINIA PELLIZZARI; MARÍA DEL CARMEN CAMBEROS; MARÍA NOEL GALARDO; SILVINA BEATRIZ MERONI; MARÍA FERNANDA RIERA

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2021

Sertoli cells (SC) are necessary to provide an adequate environment for germ cell development.SC actively metabolize glucose but most of it is converted to lactate, an energy source for germcells. SC also oxidize fatty acids (FA) to sustain their energy status. SC have numerous lipiddroplets (LD), which are thought to be the site of storage of FA. In this context, we havedemonstrated that leptin (Lep), an adipokine that is present in the testis, increasestriacylglycerols (TAGs) and LD content in SC. Several proteins such as the FA transporterFAT/CD36, the enzymes involved in the synthesis of TAGs (glycerol-phosphate-acyl-transferases(GPATs) and diacylglycerol-acyl-transferase 1 (DGAT)), and the proteins associated with LDformation (PLINs) have a role in lipid storage. The aim of this study was to analyze the effects ofLep on the expression of proteins involved in FA storage. SC isolated from 20-day-old rats werecultured and maintained under basal conditions (B) or stimulated with Lep (100 ng/ml). mRNAlevels of FAT/CD36, GPAT1-4, DGAT1 and PLIN1-3 were analyzed by RT-qPCR. Results areexpressed as mean±SD, n=3, (* p