Nitric oxide (NO), prostanoids (PR) and oxidative stress (OS) during experimental endotoxemia in fructose (F).overloaded rats

PEREDO, HORACIO A; CARRANZA, ANDREA; AZICH, ANABELLA; MAYER, MARCOS A; INGARAMO, PI; RONCO, MT; PUYÓ, ANA MARÍA; GALLEANO, MÓNICA

Santiago de Chile

Congreso; VI Meeting of SFRBM South American Group; 2009

Society of Free Radicals

Nitric oxide (NO), prostanoids (PR) and oxidative stress (OS) during experimental endotoxemia in fructose (F).overloaded rats H.A. Peredeo, M.A. Carranza, A. Azich, M. Mayer, P.I. Ingaramo, M.T. Ronco, M.A. Puyo, M. Galleano Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, IFISE, Universidad Nacional de Rosario and CONICET, Argentina The aim of this work was to study NO and PR production and OS markers during experimental endotoxemia in control and F-overloaded rats, considered a modelo f metabolic syndrome. Male Sprague-Dawley rats were grouped in controls (C, tap water, n=16) or F (F solution, 10% w/v, n=16) and treated for 15 weeks. Six hours previous to sacrifice, 8 animals from each group received LPS (4mg/kg, ip) (C-LPS and F-LPS groups) and the remaining received physiologic solution (C and F). The following parameters were measured: blood NO/hemoglobin (by ESR), plasma nitrites and nitrates (NOx), liver iNOS expression, TBARS, plasma 8-isoprostanes (8-iso, ELISA), and vascular PR production in aorta and mesenteric bed (HPLC). NoHb (UA) NOx (µ M) iNOS (UA) TBARS (µ M) 8-iso (pg/ml) C <0.5 9±0.7 2.5±1.3 1.7±0.2 184±45 F <0.5 13.1±0.6 0.6±0.3 1.53±0.06 192±26 C-LPS 19±2 264±11 68±3a 2.9±0.09 3557±400a F-LPS 11±1b 212±18a,b 55±6a,b 2.6±0.2a 2960±620a a p<0.05vs.C and F< b p<0.05vs.C-LPS (ANOVA) PLS increased the release of PR in both vascular preparations (p<0.05) in C but not in F group. Results showed that NO and PR production were lower in F-LPS rats as compared woth C-LPS group, suggesting an impaired response to LPS-challenge in F rats. Supported by UBA, CONICET and ANPCyT.