ALTERATION OF INTESTINAL INTEGRITY IN MALE C57BL/6 AND KNOCKOUT MICE FOR TNF-α RECEPTOR 1 SUBJECTED TO A HIGH-FAT DIET

GIOBERTI, FRANCO; SINGH, MACIEL; BARRANCO, MARIA MANUELA; ZECCHINATI, FELIPE; PERDOMO, VIRGINIA GABRIELA; VILLANUEVA, SILVINA STELLA MARIS; GARCÍA, FABIANA

Rosario

Congreso; XXIV Congreso y XLII Reunión Anual de la Sociedad de Biología de Rosario.; 2022

SOCIEDAD DE BIOLOGIA DE ROSARIO

Obesity is a multifactorial disease characterised by a chronic systemic inflammatory state, affecting different organs, including the small intestine. TNF-α receptor 1 (TNFR1) knockout mice are animals with an altered inflammatory response that are commonly used to study the role of this cytokine in different inflammatory pathologies. The aim of this study was to evaluate the histological modifications in the small intestine of male C57BL/6 mice and TNFR1 knockout mice subjected to a high-fat diet (HFD). Methodology: 5-week-old male C57BL/6 mice (C57) and TNFR1 knockout mice (R1KO) were randomly divided into 4 groups. The C57-C and R1KO-C groups received chow diet, and C57-HFD and R1KO-HFD groups received chow diet supplemented with 40% of fat for 16 weeks, during dietary treatment, body weight gain was assessed. At the end of treatment, an insulin tolerance test (0.75 IU/kg) was performed, then the animals were anaesthetised, sacrificed and blood obtained was used to measure biochemical parameters (cholesterol and triglycerides). Adipose and intestinal tissues were collected. Histological analysis was performed by haematoxylin-eosin staining of jejunum. Preparations were visualised by optical microscopy and analysed with ImageJ software. Statistical comparisons were performed by Student's t-test (significant at P