BECAS
SABATIE Alejandro Enrique
congresos y reuniones científicas
Título:
Effect of an arginine-based surfactant on the biophysical properties of bacterial and mammalian virus model membranes
Autor/es:
SABATIE, ALEJANDRO ENRIQUE; HERMET, MELISA; FAIT, MARÍA E.; MORCELLE, SUSANA R.; FANANI, MARÍA LAURA
Lugar:
Rosario, Santa Fé
Reunión:
Congreso; Reunión Anual de la Sociedad Argentina de Biofísica; 2022
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
Biocidal activity of cationic surfactants turns them into widely used active ingredients present in disinfectant formulations against bacteria, fungi and viruses. In this context, the aim of this work was to deepen into the biocidal mechanism of an arginine-based surfactant obtained by means of enzymatic biocatalysis in our laboratory. The effect of Nα-benzoyl-L-arginine dodecylamide (Bz-Arg-NHC12) on the biophysical properties of bacterial and mammalian virus membrane models was studied using an Escherichia coli lipid extract (ECEx) and a mammalian lipid mixture (POPC/16:0 SM/Cho 1:1:1, MVM). First, Bz-Arg-NHC12 insertion into lipid monolayers was analyzed evaluating the critical surface pressure. The surfactant was able to insert itself into highly packed preformed monolayers, up to initial surface pressures of 48 ± 2 and 47 ± 2 mN/m for ECEx and MVM respectively. Moreover, Langmuir monolayer assays carried out using different molar percentages (10 and 20 mol%) of Bz-Arg-NHC12 evidenced no effect of the surfactant on the mean molecular area at 30 mN/m. Finally, membrane stability was investigated measuring the surface pressure as a function of the mean molecular area during three compression-expansion cycles. Changes in the molecular area at 35 mN/m (maximum surface pressure measured) revealed an opposed behavior for both tested models. While MVM monolayers showed an increased stability upon Bz-ArgNH12 incorporation in a dose-dependent manner, ECEx membranes evidenced a stability reduction in the presence of Bz-Arg-NHC12. Similar results were found when the membrane compressibility modulus (Cs-1) was analyzed. Results evidenced that Bz-Arg-NHC12 was able not only to interact and insert itself in both membrane models studied, but also to modify their biophysical properties. Differential effects on membrane’s stability may have relevance in the biocidal mechanism of the surfactant in both viruses and bacteria.