Reduction of intestinal Mrp2 and P-gp activities in a high fat diet model.

BARRANCO, MARIA MANUELA; SIGAL, NICOLAS; ZECCHINATI, FELIPE; SYLVESTRE BEGNIS, MARIA; DI CARLO, BIANCA; HABIB, MARTIN JOSE; ARANA, MAITE ROCÍO; VIGNADUZZO, SILVANA; PERDOMO, VIRGINIA GABRIELA; VILLANUEVA, SILVINA STELLA MARIS; GARCÍA, FABIANA

MAR DE PLATA

Congreso; REUNIÓN CONJUNTA SAIC SAI SAFIS 2018; 2018

Metabolic syndrome (MetS) is a grave health disorder that increases the risk for cardiovascular complications and type 2 diabetes. Some drugs used in patients with MetS are substrates of intestinal multidrug resistance-associated protein 2 (Mrp2) and P-glycoprotein (P-gp), two important efflux pumps that limit the absorption of xenobiotics. Thus, their bioavailability could be affected by changes in those transporters. Because one of the major causes of MetS in humans is diet induced obesity, the aim of this study was to evaluate the effect of a high fat diet on intestinal Mrp2 and P-gp activities.C57BL/6J male mice of 5 weeks old were randomly treated for 16 weeks, with standard diet, control group (C) or were fed with standard diet enriched with 40% kcal from bovine fat, high fat diet group (HFD)) (n=10/group).Results: Mice receiving HFD showed a significant lower percentage of maximum glucose clearance in the insulin test tolerance (7.14±2.94)than C (16.18±7.53),p