INTESTINAL MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 2 (MRP2) IS DOWNREGULATED BY OXIDATIVE STRESS (OS) VIA A POSTTRANSLATIONAL MECHANISM. IMPACT ON ITS MEMBRANE BARRIER FUNCTION

ZECCHINATI, FELIPE; BARRANCO, MARIA MANUELA; TOCCHETTI, GUILLERMO NICOLÁS; DOMÍNGUEZ, CAMILA JULIANA; ARANA, MAITE ROCÍO; PERDOMO, VIRGINIA GABRIELA; LUQUITA, MACELO GABRIEL; VIGNADUZZO, SILVANA; MOTTINO, ALDO DOMINGO; GARCÍA, FABIANA; VILLANUEVA, SILVINA STELLA MARIS

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

The intestinal tract is a major site of pro-oxidant agent’s production,as a result of continuous exposure to food additives and contaminants.Homeostatic control of intestinal oxidative environmentis crucial in nutrient digestion and absorption, and barrier function.Intestinal Mrp2 is an ABC transporter that limits the absorption ofxenobiotics orally ingested, thus acting as a biochemical barrier. Wehere evaluated the short-term effect of OS on intestinal Mrp2 subcellularlocalization and its barrier function by treatment of isolatedintestinal sacs with 250 μM of tert-butyl hydroperoxide (TBH 250) for30 min (N=6). We first confirmed that TBH 250 generated OS in intestinaltissue as indicated by increasing lipid peroxidation products(+61%) and catalase (+44%) and glutathione peroxidase (+28%) activities,and by decreasing GSH content (-19%), the GSH/ GSSG ratio(-29%) and superoxide dismutase activity (-21%), respect to controls(C, p