Evaluation of the toxic effect of Zn/Cd on the antioxidant response in Glycine max and probable competition with Ca/Mg transporters

GATICA, CAMILA; GALLARDO, LAURA; CIMINARI, MARIA EUGENIA; CASÍN, NICOLÁS R.; PACHECO PABLO; PENA, LB; GIL, RAÚL A.; PÉREZ CHACA, M. VERONICA

Mendoza

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

Sociedad de Biología de Cuyo

Heavy metals pollution has produced big soil problems all around the world. Cadmium (Cd) is a heavy metal without biological functionsand also causes harmful effects at cellular and molecular levels. Zinc is an essential microelement for plants, but in excessive concentrationsit causes alterations in physiological, biochemical and molecular processes. There are soil ions that interact with Zn and possible pollutantCd, such as Calcium (Ca) and Magnesium (Mg). Metals compete to get inside, transporting and utilization in the plant; because they havesimilar chemicals characteristics and also plants absorbed them using the same transporters (IRT1-ZIP). The objective was to determinethe competition between Ca and Mg transporters with Cd and Zn, in Glycine max leaves, and its effect on antioxidant and prooxidantparameters. Leaves were obtained after 10 days of plant development under hydroponics in Hoagland´s nutrient solution conditions andsubjected to contamination with two ions (Zn and Cd) during 6 days. The ZnCl2 concentrations used in the study were: 0, 0.6 and 4.8 mMand 40 M of CdCl2, as a constant concentration. The measured parameters were photosynthetic pigments (Chlor) and carotenes (Car) andCa and Mg endogen content. CAT and APX antioxidant enzymes, MDA and H2O2 content were measured as oxidative response. Resultsshowed a significant decrease in Car and Chlor in Zn (4.8 mM)/ Cd (p