Desmoglein-4 deficiency impairs humoral immunity induced by OVA intradermal administration

MICHEL MARÍA CECILIA; TRONCOSO MARIANA; SÁNCHEZ MARÍA BELÉN; MORENO SOSA MARÍA TAMARA; NEIRA FLAVIA JUDITH; CAMPO-VERDE-ARBOCCÓ FIORELLA; SOAJE MARTA; SÁNCHEZ MARÍA VICTORIA; TRONCOSO A; MOISO A; PIETROBON ELISA OLIVIA; INNOCENTI BADANO ALICIA CAROLINA; JAHN GRACIELA ALMA; VALDEZ SUSANA RUTH; MACKERN OBERTI JUAN PABLO

Congreso; XXXIX Reunión Científica Anual Sociedad de Biología de Cuyo; 2021

Desmogleins (Dsg) are transmembrane proteins located in desmosomes and play a critical role in the cell-cell adhesion of epithelial cells. Dsg-4 deficiency is associated with sharp hair loss in humans, mice, and rats. Recently, we have reported a clear link between Dsg-4 deficiency, exacerbated inflammation, and psoriasis-like lesions after imiquimod (IMQ) administration. However, the role of Dsg-4 in humoral immunity has not been addressed. Therefore, this work aimed to study the impact of Dsg-4 deficiency in OVA-specific IgG induction. For this purpose, OFA hr/hr deficient in Dsg-4, and wild- type Sprague-Dawley (SD) rats were inoculated intradermally with 20μg/40μl OVA/PBS. IMQ (100 mg), or Vaseline (Vas) (100mg), was administered topically after four hours from OVA injection and for the following three days. Two weeks later, serum samples were obtained by maxillary puncture to determined OVA-specific IgG levels by ELISA. Surprisingly, OFA rats displayed lower OVA-specific IgG levels compared to SD group (OFA 0.118±0.095 vs SD 0.745±0.171, t test, p