Role of Angiotensin II type 2 receptor (AT2R) in microtubule response to ischemic injury

RIVABELLA MATKINS TOMAS; FUSSI M. FERNANDA; ALEJANDRO, PARIANI; FAVRE CRISTIAN ; MOLINAS, SARA M.; LAROCCA, M. CECILIA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC, SAFIS, SAI 2022; 2022

Sociedad Argentina de Investigación Clínica y Sociedad Argentina de Fisiología

Ischemic acute kidney injury (IAKI) leads to alterations of the microtubulecytoskeleton and loss of epithelial integrity in proximal tubularcells. AT2R pre-stimulation elicits renoprotective effects in IAKI, preservingtubular cell polarity. Primary cilia are microtubule based sensoryorganelles whose stability correlates with their acetylated-alphatubulin levels (ac-tub). Because microtubules and, specifically, theprimary cilia modulate cell homeostasis, we used a cell-model ofIAKI to examine AT2R role in microtubule and primary cilia responseto ischemic injury. Methods: polarized MDCK cells were incubated inserum free media containing antimycin A 10 μM and 2-deoxyglucose10 mM by 90 min (Ischemia, I) and re-incubated with full media for 2h (I-reperfusion, IR) at 37 °C. AT2R was stimulated with CGP42112A0.1 μM (CG) beginning 24h before damage. Cell viability was testedby Trypan Blue exclusion. Microtubule integrity and cilia morphologywere analyzed by confocal microscopy and relative ac-tub by westernblot. Results are expressed as media +/- SE. *p