Sex differences in the response to ischemic acute kidney injury and in urinary NGAL biomarker

FUSSI M. FERNANDA; RIVABELLA MATKINS TOMAS; BULACIO ROMINA; HAZELHOFF M. HERMINIA; MARQUEZ SUSANA; CAMPAGNO ROMINA; DE VUONO DANIEL; MASSONI CLAUDIA; RIERA JM; LLOI SUSANA; MOLINAS JORGE; BRANDONI ANABEL; MONASTEROLO, LILIANA A.; LAROCCA MC; MOLINAS, SARA M.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC, SAFIS, SAI 2022; 2022

Sociedad Argentina de Investigación Clínica y Sociedad Argentina de Fisiología

One of the main causes of acute kidney injury (AKI) is ischemia-reperfusion (IR). Animal studies have shown that female sexprotects against the development of ischemic renal injury. Neutrophilgelatinase-associated lipocalin (NGAL) is increasingly secretedby tubular cell during AKI, constituting a promising diagnostic andprognostic biomarker. However, there are limited studies in femaleanimals and sex differences in the urinary excretion of this biomarkerduring AKI are not clear. Our aim was to characterize the renalresponse to IR damage in males and females rats, and evaluatethe correlation with uNGAL levels. Male (MIR) and female (HIR)Wistar rats (n=6 per group) underwent 40 min of unilateral renalischemia followed by 1 day of reperfusion. Controls underwent shamoperation (MC and HC). Functional and histological studies werewas evaluated in renal tissue by Western Blot. uNGAL was analyzedby Western Blot. IR damage produced a decrease in glomerular filtrationrate, estimated by creatinine clearance, that was greater inMIR (-38% p