Extracellular vesicles as nanocarrier for therapeutic use in demyelinating diseases

MATTERA, VANESA; PASQUINI, JM; CORREALE, J

Virtual

Congreso; Congress in Translational Medicine; 2021

Facultad de Farmacia y Bioquimica - UBA

EVs capacity for biologic information transfer makes them an attractive tool as therapeutic agents for nanodelivery of different molecules to target cells. They are able to cross the blood brain barrier, their structure is biocompatible and the cargo is protected from degradation, which increases molecule stability, solubility and bioavailability. Previous work by our group has shown the pro-differentiating effects of apoTransferrin (aTf) on oligodendroglial cells in vivo and in vitro. The remyelinating effect of aTf was also observed in a model of hypoxia/ischemia where aTf was intranasally administered. EVs were isolated from mouse plasma and characterized by Western blot, dynamic light scattering and scanning electron microscopy.Isolated EVs containing the Tf receptor 1 (TfR1) were loaded with aTf, specifically through binding to TfR1, using passive cargo-loading strategies which rendered EVTf loaded particles (Mattera et al., 2020). In this work, we show the promaturation effect of the EV-Tf system on oligodendroglial primary cultures as assessed through different cell markers. These results show EVs as potential nanovehicles of aTf to be delivered into the CNS parenchyma and pave the way for further studies into their possible clinical application in the treatment of demyelinating diseases